Pensacola Spore Syringe

(109 customer reviews)


Pensacola cubensis comes all the way from the sunshine state, Florida US. The mycelium is very aggressive and grows quickly as well. The fruits resemble the South American strains with their spade like shape. It does produce some aborts, but as we understand this is not really problematic for many of our customers.

The spores are available in vials and syringes.

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Pensacola 20cc magic mushroom spore syringes are produced under sterile conditions and should be kept dark and refrigerated (2-8°C) to increase the shelf life of the cubensis spores.


  • 1x 20cc Pensacola spore solution in a 20ml syringe
  • 1x sterile needle
  • 4x Alco prep


Special Solution

The Pensacola cubensis spores are dissolved in a specially developed organic solution to promote the quality of the spores.

Compared to water based spore solutions;  you will get larger yields, stronger mycelium and faster colonization because of this special spore solution.

Why these syringes are better than other spore syringes

  • Spores are 100% free from contaminants and they are very contaminant resistant after leaving the syringe.
  • Improved shelf life. Over 6 months longer; now 18 months.
  • Spores are not sticking to sides of the syringes anymore, thus easier to handle and more grow parts per milliliter (500 gpp ml).
  • Contains nutrients to ‘kickstart’ the spores when inoculating.
  • Solution boosts strength of mycelium.
  • Faster colonization of substrate and cakes.


How to use the Pensacola spore syringe

The Pensacola spore solution is held inside the syringe by a luer lock stopper on the end where the needle goes. With this luer lock stopper attached makes it easier to transport and to store the Pensacola spore syringe.

Make sure to work as hygienic as possible. Wear a face mask and sterile gloves and/or disinfect your hands. Hold the Pensacola spore syringe in front of you with the luer lock stopper pointing upwards. Remove the luer lock stopper by screwing it counter-clockwise. Take the needle out of the sterile package and screw it on the Pensacola spore syringe. Shake the spore syringe vigorously for ten seconds before you remove the protective cap from the needle.

To proceed from here, there are several methods to choose from:

Dropping the spore solution onto the substrate

After having prepared your own substrate or cakes you can drop the Pensacola spore solution directly from the syringe onto these. Press the plunger slowly so that drops of spore solution will come out of the needle. Every drop that will be in contact with the substrate will be a potential mycelium growth point, so spread out these drops all over the substrate to evenly colonize the substrate with mycelium.

How many cakes can you make with spore syringe?

One 20cc spore syringe can easily colonize 6 liters / 13 pints of substrate.

Injection through injection ports

This is almost identical as dropping the spore solution onto the substrate. Open the injection port by pulling back the port protection cap. Use a alco prep to sterilize the injection port entry. Pierce the opening with the needle. Now drop the spore solution evenly over the substrate inside the spawn bag or grow box. After the Pensacola spore solution is injected, remove the syringe and close the injection port with the port protection cap.

Study spores and research using a microscope

Seen from a scientific point of view; all Psilocybe cubensis are alike. But they do differ slightly in appearance and fruiting speed (phenotype). There are numerous Psilocybe cubensis that have different phenotypes. These different cubensis are not considered subspecies, but different strains. The Pensacola is a strain of Psilocybe cubensis.

The differences in phenotype can be seen with the naked eye, but the true beauty lies under the microscope. The wondrous world of the spores. Many of our customers are scientists or enthusiasts, doing research on these differences in a homemade laboratory. After having prepared your microscope slide, drop some of the Pensacola spore solution on it. Cover the specimen with a microscope covering glass. The best magnification for Psilocybe cubensis and Panaeolus cyanescens spore research is a magnification of 1000x or higher.

Using liquid culture growth medium with the spore syringe

The special solution in which the Pensacola cubensis spores are dissolved, kickstarts the growth of the mycelium. We do not recommend the use of liquid culture growth medium to get stronger mycelium. If you desire to produce liquid cultures, we recommend to choose for the professional Pensacola spore vial rather than the Pensacola spore syringe. If you want to experiment with this Pensacola spore syringe and liquid culture growth media, we’d love to hear your feedback on your results.

How to store a spore syringe

The Pensacola spore syringe should be stored refrigerated in a temperature between 2°C – 8°C or 35°F – 46°F. The shelf life of the spore syringe is at least 12 months after they leave our warehouse.

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